Alessandro d’Agostini began his musical studies at the age of five. After graduating in piano, conducting, composition, and computer music, he furthered his studies with Giorgio Nottoli (composition for computer music) at the Accademia Chigiana in Siena and with Konstantin Bogino, the Tchaikovsky Trio (piano and chamber music). Conducting was taught by Piero Bellugi and Massimo de Bernart, the latter of whom he was assistant.
In 2002, he made his operatic debut in Rossini's William Tell (an “As.Li.Co.” production, Milan) at the Circuito Lombardo theaters and at the Teatro Comunale in Bologna. In the following years, he conducted other “As.Li.Co.” productions. such as Gluck's Orfeo ed Euridice, Donizetti's L'Elisir d'amore, and Puccini's Madama Butterfly. In 2003, he conducted the Toscanini Foundation Symphony Orchestra of Parma in the first modern performance of Leonardo Leo's “commedia per musica” Lo Matremmonio annascuso, based on his own critical edition.
Alongside his conducting work, he also engages in musical research, particularly on 18th-century opera: his critical editions and first modern performances of Cherubini's Iphigenia in Aulis and Leo's L'Olimpiade are notable.
In the years since, he has undertaken an intense artistic career. Among his most notable performances are: Falstaff at the Teatro Verdi in Busseto; Il Trovatore at the Macerata Opera – Sferisterio; Cilea's L'Arlesiana at the Teatro delle Celebrazioni in Bologna and the Teatro Sociale in Mantua; Verdi's Aida and Otello at the National Theatre in Tirana, Albania; His debuts at the Teatro Comunale di Bologna (Leo's L'Olimpiade); at the Teatro dell'Opera di Roma during the Summer Season at the Baths of Caracalla, in Adam's Giselle, choreographed by Carla Fracci; Rigoletto (with Jessica Pratt as Gilda, Como – Città della Musica Festival); and Donizetti's Figlia del Reggimento in the Circuito Lombardo (with Yolanda Auyanet and Gianluca Terranova). He made his debut with L'Elisir d'amore at the Teatro Lirico di Cagliari, where he returned for Tosca and numerous symphony concerts; he also made his highly successful debut at the Maggio Musicale Fiorentino conducting Rossini's Il Barbiere di Siviglia.
In the symphonic field, he has conducted the Maggio Musicale Fiorentino Orchestra, the Toscanini Foundation Symphony Orchestra of Parma, the Toscanini Philharmonic Orchestra, the Orchestra of the Teatro Lirico of Cagliari, the Pomeriggi Musicali of Milan, the G.F. Malipiero Venetian Philharmonic Orchestra, the Italian Philharmonic Orchestra, the Macedonian Philharmonic, the Russian National Orchestra, the Evgenij Svetlanov State Academic Symphony Orchestra, the Moscow State Symphony Orchestra, the St. Petersburg Academic Philharmonic Orchestra, and the “Novaya Rossiya” State Symphony Orchestra.
From 2011 to 2013, he was Principal Guest Conductor at the Makedonska Opera i Balet (National Opera Theatre of the Republic of Macedonia), where he conducted productions of Tosca, Turandot, Attila, Aida, Lucia di Lammermoor, The Barber of Seville, and La Traviata.
In 2012, he made his debut at the Tenerife Auditorium in Mozart's Die Zauberflöte; he returned there in 2022 to conduct Un Ballo in Maschera.
He is frequently invited as a Guest Conductor to Russia, conducting concerts with major orchestras: the Russian National Orchestra, the Svetlanov State Academic Symphony Orchestra, the Moscow State Symphony Orchestra, and the “Novaya Rossiya” State Symphony Orchestra. Among his many numerous performances of masterpieces, such as Orff's Carmina Burana and Mussorgsky/Ravel's Pictures at an Exhibition, in the Great Hall of the Tchaikovsky Conservatory and at the International House of Music in Moscow, always met with enthusiastic acclaim.
In recent years, he has been regularly invited to the Maggio Musicale Fiorentino to conduct various opera and ballet productions, including Adam's Giselle, Auber's Fra Diavolo, Beethoven's Fidelio, L'Elisir d'amore, Rossini's The Barber of Seville, and La Cenerentola, Verdi's Aida, and symphonic programs.
He also made his debut at the Teatro Massimo in Palermo in Donizetti's L'Elisir d'amore, where he returned the following year to replace Daniel Oren in Leoncavallo's Pagliacci, achieving great personal success. He also inaugurated the Jinan and Zibo Opera Houses in China with Mozart's Don Giovanni. Cavalleria rusticana at the Priamar Fortress in Savona, leading the Orchestra and Chorus of the Teatro Carlo Felice in Genoa; The Barber of Seville at the Teatro Regio in Parma; Bellini's La Sonnambula at the Teatro delle Muse in Ancona; La Traviata, Rigoletto, Lucia di Lammermoor, and Un Ballo in Maschera at the Teatro Comunale Pavarotti in Modena; and his debut in the 2020 Symphony Season of the Arena di Verona Foundation (Teatro Filarmonico).